Nanoliter droplet vitrification for oocyte cryopreservation
نویسندگان
چکیده
منابع مشابه
Nanoliter droplet vitrification for oocyte cryopreservation.
AIM Oocyte cryopreservation remains largely experimental, with live birth rates of only 2-4% per thawed oocyte. In this study, we present a nanoliter droplet technology for oocyte vitrification. MATERIALS & METHODS An ejector-based droplet vitrification system was designed to continuously cryopreserve oocytes in nanoliter droplets. Oocyte survival rates, morphologies and parthenogenetic devel...
متن کاملNumerical simulation of cooling rates in vitrification systems used for oocyte cryopreservation.
Oocyte cryopreservation is of key importance in the preservation and propagation of germplasm. Interest in oocyte cryopreservation has increased in recent years due to the application of assisted reproductive technologies in farm animals such as in vitro fertilization, nuclear transfer and the need for the establishment of ova/gene banks worldwide. However, the cryopreservation of the female ga...
متن کاملSimplified EM Grid Vitrification Is a Convenient and Efficient Method for Mouse Mature Oocyte Cryopreservation
This study was performed to evaluate the efficiency of simplified EM grid vitrification, skipping the step of removing the cryoprotectant (5.5M EG + 1.0M sucrose) droplet on the grid after loading oocytes, compared to conventional cryopreservation protocols for mouse mature oocytes. Firstly, the recovery, survival, fertilization and hatching rates of simplified EM grid vitrification were compar...
متن کاملNumerical analysis to determine the performance of different oocyte vitrification devices for cryopreservation.
Vitrification is widely used for cryopreservation of oocytes. The present study has built the theoretical models of four vitrification systems (micro-droplet, open pulled straws, quartz micro-capillary and cryotop), and performed numerical analysis to predict the cooling rates. The numerical analysis shows that the average cooling rate of the cryotop system was higher than those of other three ...
متن کاملOocyte cryopreservation.
OBJECTIVE To review historical and contemporary advances in oocyte-cryopreservation techniques and outcomes. DESIGN Publications related to oocyte cryopreservation were identified through MEDLINE and other bibliographic databases. CONCLUSION(S) Oocyte cryopreservation can be used as an adjunct to conventional IVF and as an option for fertile women to electively cryopreserve their gametes. R...
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ژورنال
عنوان ژورنال: Nanomedicine
سال: 2012
ISSN: 1743-5889,1748-6963
DOI: 10.2217/nnm.11.145